NANOG Is Multiply Phosphorylated and Directly Modified by ERK2 and CDK1 In Vitro

NANOG Is Multiply Phosphorylated and Directly Modified by ERK2 and CDK1 In Vitro

Blog Article

NANOG is a divergent homeobox protein and a core component of the transcriptional circuitry that sustains pluripotency and self-renewal.Although 1200D Nylon Horse Rug Combo (200g fill) NANOG has been extensively studied on the transcriptional level, little is known regarding its posttranslational regulation, likely due to its low abundance and challenging physical properties.Here, we identify eleven phosphorylation sites on endogenous human NANOG, nine of which mapped to Razor single amino acids.To screen for the signaling molecules that impart these modifications, we developed the multiplexed assay for kinase specificity (MAKS).

MAKS simultaneously tests activity for up to ten kinases while directly identifying the substrate and exact site of phosphorylation.Using MAKS, we discovered site-specific phosphorylation by ERK2 and CDK1/CyclinA2, providing a putative link between key signaling pathways and NANOG.